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Palsson, Bernhard Ø; Herring, Christopher D; Raghunathan, Anu; Honisch, Christiane; Patel, Trina; Applebee, M Kenyon; Joyce, Andrew R; Albert, Thomas J; Blattner, Frederick R; van den Boom, Dirk; Cantor, Charles R
Nature genetics, 12/2006, Letnik: 38, Številka: 12Journal Article
We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts.
