Abstract This study aims to screen out the best spectrum possible by study the effect of spectral treatment on biological habit of Scopula subpunctaria Herrich-Schaeffer, and provided a reference for the integrated management of S. subpunctaria. Total 6 groups (90) of adult S. subpunctaria , 9 groups (450) of eggs, 3 groups (300) of pupae, treated with continuous light treatment under room condition from 7:00 pm to 7:00 am every day at 370-375 nm or 420-425 nm, respectively, the control group (45 adult S. subpunctaria ) without light treatment. The spawning amount and longevity of adults, the hatching rate of eggs and the eclosion rate of pupae in each group were recorded. The results showed that the total egg quantity and oviposition rate had been effected significantly under illumination treatments. The egg number in every female adult reduced 28.9 and 53.5% under 370~375 nm and 420~425 nm illumination, respectively, compared with the control group, and showed a significant difference level; while the average oviposition rate reduced 11.10 and 15.90% respectively. Meanwhile, the pre-oviposition period and oviposition period were affected by the two illumination treatments. There was no significant effect on the adult longevity of S. subpunctaria nor adult longevity in group feeding nor paired feeding conditions. The egg quantity in groups feeding was higher than that in pairs, and 420~425 nm was reached a significant level contrast to the control deal. The spectral treatments of 370~375 nm and 420~425 nm have effects on the biological parameters of S. subpunctaria , and the latter has significant effects. In conclusion, the light sources can be provided in the tea garden at night (from 7:00 pm to 7:00 am) before the emergence of the adult S. subpunctaria to interfere its population development.