This study focuses on the health risk assessment of arsenic (As), copper (Cu), zinc (Zn), and selenium (Se) concentrations in seafood species commonly consumed in the northwestern region of Croatia. By measuring the concentrations of these elements coupled with data on seafood consumption, the health risks were evaluated using Target Hazard Quotients and Hazard Indexes. The results indicate a slightly increased health risks linked to seafood consumption for As, Cu and Zn in some of the tested seafood species. The findings of this study highlight the critical need for stronger food quality control measures, especially targeting certain types of seafood. Display omitted •The report analysed the health risks associated with the presence of As, Cu, Zn and Se in seafood in the Adriatic Sea.•The calculated THQs and HIs point out to a slightly elevated health risks attributable to the presence of the tested metals in seafood.•The study provides a valuable roadmap for future research and regulatory actions in the field of seafood safety.