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Vincendeau, Estelle; Wei, Wenming; Zhang, Xuefei; Planchais, Cyril; Yu, Wei; Lenden-Hasse, Hélène; Cokelaer, Thomas; Pipoli da Fonseca, Juliana; Mouquet, Hugo; Adams, David J; Alt, Frederick W; Jackson, Stephen P; Balmus, Gabriel; Lescale, Chloé; Deriano, Ludovic
Nature communications, 06/2022, Letnik: 13, Številka: 1Journal Article
SHLD1 is part of the Shieldin (SHLD) complex, which acts downstream of 53BP1 to counteract DNA double-strand break (DSB) end resection and promote DNA repair via non-homologous end-joining (NHEJ). While 53BP1 is essential for immunoglobulin heavy chain class switch recombination (CSR), long-range V(D)J recombination and repair of RAG-induced DSBs in XLF-deficient cells, the function of SHLD during these processes remains elusive. Here we report that SHLD1 is dispensable for lymphocyte development and RAG-mediated V(D)J recombination, even in the absence of XLF. By contrast, SHLD1 is essential for restricting resection at AID-induced DSB ends in both NHEJ-proficient and NHEJ-deficient B cells, providing an end-protection mechanism that permits productive CSR by NHEJ and alternative end-joining. Finally, we show that this SHLD1 function is required for orientation-specific joining of AID-initiated DSBs. Our data thus suggest that 53BP1 promotes V(D)J recombination and CSR through two distinct mechanisms: SHLD-independent synapsis of V(D)J segments and switch regions within chromatin, and SHLD-dependent protection of AID-DSB ends against resection.
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Vir: Osebne bibliografije
in: SICRIS
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