Eccentric overload training was recently applied in movement variability analysis. The in-court response during training and match varies according to playing position, however, regarding eccentric-overload training, this dependency is scarcely explored. The aim of this study was to examine the influence of playing positions on physical parameters in strength exercises using eccentric-overload device. Nine under-16 regional level basketball players were recruited for this study. Participants were grouped according to their playing positions: point guards (n=3; height = 171.1 ±3.2 cm), wings (n=3; height = 170.7±4.1 cm) and power forwards (n=3; height = 188.2±4.1 cm). Point guards showed higher mean velocity (Vm) and Approximate entropy (ApEn) in all axis in Side. Wings presented higher ApEn in all axis in Back, while power forwards obtained a higher Vm. Finally, power forwards showed higher Vm and ApEn in all axis in Lateral.