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  • In vitro gas profile of dai...
    Afdal, M; Darlis, D; Adriani, A

    IOP conference series. Earth and environmental science, 04/2020, Letnik: 492, Številka: 1
    Journal Article

    The aim of this study was to revealthe in vitro gas profile of basal diet added with Coleus amboinicus lour (CAL). The basal diet was added with CAL extracted with different solvent of each water and ethanol. There were four treatments of this experiment namely P0: basal diet (control), P1: basal diet added with 2 % of CAL powder, P2:basal diet added with 2 % of CAL extracted with water, P3 basal diet added with 2 % of CAL extracted withethanol. Each sample was incubated for 48 h following the Reading Gas technique methods with slight modification. Gas was collected at 2, 4, 6, 8, 12, 18, 36 and 48 h. The parameters measured were the total gas production (P), potential gas production (b) and rate of gas production (c). The results of this experiment showed that P and b were significant (P<0.05) different. The total gas production was 122.16, 94.78, 122.34 and 62.34 ml/g for P0, P1, P2 and P3 respectively and b was 124.88, 100.13, 117.05 and 80.76 ml/g for P0, P1, P2 andP3 respectively. The value of c was not significant (P>0.05) among four treatments. The value of c was 0.0625, 0.0525, 0.0525 and 0.0425 for P0, P1, P2 andP3 respectively. It could be concluded that the use of water to extract CAL was the best among four treatments.