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Kwon, Eunju; Pathak, Deepak; Dahal, Pawan; Tandukar, Sudarshan; Jung, Hyun Suk; Kim, Woe-Yeon; Kim, Dong Young
Cell reports, 04/2022, Letnik: 39, Številka: 3Journal Article
In Arabidopsis, GIGANTEA (GI), together with the blue-light receptors ZTL, LKP2, and FKF1, regulates degradation of the core clock protein TOC1 and the flowering repressor CDFs, thereby controlling circadian oscillation and flowering. Despite the significance of GI in diverse plant physiology, its molecular function is not much understood because of technical problems in protein preparation and a lack of structural information. Here, we report the purification of the GI monomer and the crystal structure of the GI/LKP2 complex. The crystal structure reveals that residues 1–813 of GI possess an elongated rigid structure formed by stacking hydrophobic α-helices and that the LOV domain of LKP2 binds to the middle region of the GI (residues 563–789). Interaction analysis further shows that LOV homodimers are converted to monomers by GI binding. Our results provide structural insights into the regulation of the circadian clock and photoperiodic flowering by GI and ZTL/LKP2/FKF1. Display omitted •GI possesses an elongated rigid structure formed by stacking hydrophobic α-helices•GI forms a heterodimer with the LOV domains of ZTL, LKP2, and FKF1•The LOV domain binds to the middle region of GI at residues 563–789•LOV homodimer is disrupted by GI binding GIGANTEA (GI) and blue-light receptors contribute to plant circadian rhythm and flowering. Kwon et al. describes the crystal structure of GI in complex with the LOV domain of LKP2 from Arabidopsis thaliana. This reveals the overall structure of GI and its interactions with the LOV domains.
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