The biosurfactant-producing bacterial isolate was isolated from an oil-contaminated water sample. This study aimed to determine the production of biosurfactant and its effect on the plant growth via the biosorption of some heavy metals. The isolate was identified by 16S rRNA gene sequence analysis as Bacillus megaterium ATTC 14581. The surface activity of the produced biosurfactant was determined. The biosurfactant was recovered from the cell-free culture of a bacterial strain at 1.5 g/L. The extracted biosurfactant shows potential stability towards some factors, like temperature and salinity. The FTIR analysis confirmed the production of surfactin biosurfactant. The impact of B. megaterium biosurfactant on Pb and Ar significantly increases morphological features, proline, and antioxidant enzymes, while a significant decrease in lipid peroxidation, H2O2, and O2.