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Ang, Lay Teng; Nguyen, Alana T; Liu, Kevin J; Chen, Angela; Xiong, Xiaochen; Curtis, Matthew; Martin, Renata M; Raftry, Brian C; Ng, Chun Yi; Vogel, Uwe; Lander, Angelika; Lesch, Benjamin J; Fowler, Jonas L; Holman, Alyssa R; Chai, Timothy; Vijayakumar, Siva; Suchy, Fabian P; Nishimura, Toshinobu; Bhadury, Joydeep; Porteus, Matthew H; Nakauchi, Hiromitsu; Cheung, Christine; George, Steven C; Red-Horse, Kristy; Prescott, Joseph B; Loh, Kyle M
Cell, 07/2022, Letnik: 185, Številka: 14Journal Article
Stem cell research endeavors to generate specific subtypes of classically defined "cell types." Here, we generate >90% pure human artery or vein endothelial cells from pluripotent stem cells within 3-4 days. We specified artery cells by inhibiting vein-specifying signals and vice versa. These cells modeled viral infection of human vasculature by Nipah and Hendra viruses, which are extraordinarily deadly (∼57%-59% fatality rate) and require biosafety-level-4 containment. Generating pure populations of artery and vein cells highlighted that Nipah and Hendra viruses preferentially infected arteries; arteries expressed higher levels of their viral-entry receptor. Virally infected artery cells fused into syncytia containing up to 23 nuclei, which rapidly died. Despite infecting arteries and occupying ∼6%-17% of their transcriptome, Nipah and Hendra largely eluded innate immune detection, minimally eliciting interferon signaling. We thus efficiently generate artery and vein cells, introduce stem-cell-based toolkits for biosafety-level-4 virology, and explore the arterial tropism and cellular effects of Nipah and Hendra viruses.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Vir: Osebne bibliografije
in: SICRIS
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