E-viri
Recenzirano
-
Manai, R.; Habchi, M.; Kamouni-Belghiti, D.; Persuy, M.A.; Rousseau, L.; Possas Abreu, M.; Grebert, D.; Badonnel, K.; Bergonzo, P.; Pajot-Augy, E.; Sanz, G.; Scorsone, E.
Sensors and actuators. B, Chemical, 01/2017, Letnik: 238Journal Article
•Immobilization of two olfactory receptors (M71 and OR7D4) onto diamond transducers is proposed.•Two different grafting procedures are monitored by EIS on BDD surfaces and micro-cantilevers.•Odorant detection is suggested in liquid phase using Laser Doppler read-out system.•M71 shows a good sensitivity to acetophenone and selectivity against 2-octanone.•OR7D4 sensor shows a good sensitivity to androstenone with selectivity against acetophenone and 2-octanone. Two olfactory receptors (ORs), mouse M71 and chimpanzee OR7D4, were immobilized onto synthetic diamond transducers surfaces. 6His tagged M71 (6His-M71) was grafted using covalent attachment of nitriloacetic acid (NTA) as chelating agent, which could bind the 6His tagged receptor through nickel ions. OR7D4 was grafted through covalent bonding of hexanoic acid radical on diamond followed by EDC/NHS peptidic coupling to the receptor. Both grafting procedures were monitored by electrochemical impedance spectroscopy (EIS) on boron doped diamond (BDD) electrodes. Then the grafting protocols were applied to the surface of bulk diamond micro-cantilevers. The resulting sensors were assessed for odorant detection in the liquid phase using a Laser Doppler read-out system. The 6His-M71 based sensor was found to exhibit a good sensitivity to acetophenone, with a typical frequency shift near 100Hz for 1μM exposure, with a good selectivity against negative control 2-octanone. The OR7D4 based sensor showed a sensitivity of 200Hz for exposures to 1 or 10μM androstenone with a good selectivity against both non-ligands acetophenone and 2-octanone.
Avtor
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.