E-viri
Recenzirano
Odprti dostop
-
Low, Jian Hui; Li, Pin; Chew, Elaine Guo Yan; Zhou, Bingrui; Suzuki, Keiichiro; Zhang, Tian; Lian, Michelle Mulan; Liu, Meng; Aizawa, Emi; Rodriguez Esteban, Concepcion; Yong, Kylie Su Mei; Chen, Qingfeng; Campistol, Josep M.; Fang, Mingliang; Khor, Chiea Chuen; Foo, Jia Nee; Izpisua Belmonte, Juan Carlos; Xia, Yun
Cell stem cell, 09/2019, Letnik: 25, Številka: 3Journal Article
Human pluripotent stem cell-derived kidney organoids recapitulate developmental processes and tissue architecture, but intrinsic limitations, such as lack of vasculature and functionality, have greatly hampered their application. Here we establish a versatile protocol for generating vascularized three-dimensional (3D) kidney organoids. We employ dynamic modulation of WNT signaling to control the relative proportion of proximal versus distal nephron segments, producing a correlative level of vascular endothelial growth factor A (VEGFA) to define a resident vascular network. Single-cell RNA sequencing identifies a subset of nephron progenitor cells as a potential source of renal vasculature. These kidney organoids undergo further structural and functional maturation upon implantation. Using this kidney organoid platform, we establish an in vitro model of autosomal recessive polycystic kidney disease (ARPKD), the cystic phenotype of which can be effectively prevented by gene correction or drug treatment. Our studies provide new avenues for studying human kidney development, modeling disease pathogenesis, and performing patient-specific drug validation. Display omitted •Modulation of WNT generates vascularized and segmentally patterned kidney organoids•Single-cell analysis identifies a non-conventional origin of renal vasculature•Structural and functional maturation of kidney organoids occurs upon implantation•Cystic kidney organoids from ARPKD iPSCs enable personalized drug validation Human PSC-derived organoids are an amenable platform for understanding human development and diseases despite numerous limitations. Low et al. establish a versatile platform for generating vascularized and patterned kidney organoids. Using this platform, they identified a non-conventional origin of renal vasculature and recapitulated ARPKD cystogenesis in vitro.
Avtor
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.