Display omitted •The microchannels emulsifier is able to form a rather stable water–oil emulsion.•WiDE resulted stable after 2 h running into the high pressure common rail system.•WiDE emulsion was successfully used in an optical diesel engine.•WiDE implied a reduction in PM and NOx compared to that resulting from diesel oil.•The 2D chemiluminescence technique highlights a reduction in soot formation. The paper reports the results of an experimental investigation carried out on a prototype optically accessible compression ignition engine fueled with water in diesel emulsion (WiDE) and Diesel only. The effect of WiDE on combustion process evolution and exhaust emissions was investigated through standard engine benchmark and optical diagnostics. 2D chemiluminescent emission measurements centered at 690 nm were carried out during the whole combustion process to discriminate the soot emission from other excited chemical species. The emulsion was produced through a prototype designed microchannels emulsifier that can also work inline. The water concentration was 9.1%v with a small amount (0.2%v) of nonionic surfactant (SPAN80) used to stabilize the emulsion. Tests were performed comparing combustion and exhaust emissions of the reference commercial diesel fuel to the WiDE. For any investigated fuel and operating point, engine tests were carried out changing the injection interval to achieve the same chemical energy as the reference diesel (935 J/str). Compared to Diesel, the WiDE induced an increase in ignition time, enhancing the air/fuel mixing with a simultaneous reduction in both PM and NOx. The digital imaging and 2D chemiluminescence techniques highlighted a reduction in soot formation without significant changes on the soot oxidation rate. The results suggest the use of WiDE as a reliable method to improve NOx-soot trade-off of CI engines.