Prosocial behavior, helping others in need in particular, occurs preferentially in response to the perceived distress of one’s own group members or ingroup. To investigate the development of ingroup bias, neural activity during a helping test was analyzed in adolescent and adult rats. Although adults selectively released trapped ingroup members, adolescent rats helped both ingroup and outgroup members, suggesting that ingroup bias emerges in adulthood. Analysis of brain-wide neural activity, indexed by expression of the early-immediate gene c-Fos, revealed increased activity for ingroup members across a broad set of regions previously associated with empathy. Adolescents showed reduced hippocampal and insular activity and increased orbitofrontal cortex activity compared to adults. Non-helper adolescents demonstrated increased amygdala connectivity. These findings demonstrate that biases for group-dependent prosocial behavior develop with age in rats and suggest that specific brain regions contribute to prosocial selectivity, pointing to possible targets for the functional modulation of ingroup bias. Display omitted •Adolescents rats — but not adult rats — helped outgroup members, releasing them from a trap•Neural activity was higher for ingroup members across some regions independent of age•Activity was modulated by age in the insula, hippocampus, hypothalamus, and vOFC•Adolescent “non-openers” showed increased amygdala connectivity Behavioral neuroscience; Cellular neuroscience;