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Guimarães, Janaína Valadares, RN, PhD; Costa, Fabiana Bettanin; Andrade, Wanessa Machado; Vêncio, Eneida Franco, RN, PhD; Salge, Ana Karina Marques, RN, PhD; Siqueira, Karina Machado, RN, Master in Nursing; Texeira, Vicente de Paula Antunes, MD, PhD
Annals of diagnostic pathology, 10/2011, Letnik: 15, Številka: 5Journal Article
Abstract The objective of the study was to identify mastocytosis in the chorionic epithelium of the uterine cervix in HIV-infected and non–HIV-infected women in autopsy specimens using histochemistry and immunohistochemistry techniques. Sixteen cervical tissue specimens were collected, of which 10 (62.50%) were from HIV-infected women. Histochemical and immunohistochemical techniques were used to evaluate mast cell density using Giemsa stain and anti-mast cell tryptase and anti-mast cell chymase antibodies, respectively. The study of the sheets and counting of mast cells with blue (Giemsa) or brown staining (anti-mast cell tryptase or chymase antibodies) were performed by 3 examiners, and 10 consecutive fields were examined under a light microscope at 400× magnification. A significant difference was found in mast cell density in the chorionic epithelium of the cervix in HIV-infected compared with non–HIV-infected women. The present study may contribute to the characterization of genital mucosa abnormalities and help better understand the potential role of mast cells in HIV-infected women.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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