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Maitituoheti, Mayinuer; Keung, Emily Z.; Tang, Ming; Yan, Liang; Alam, Hunain; Han, Guangchun; Singh, Anand K.; Raman, Ayush T.; Terranova, Christopher; Sarkar, Sharmistha; Orouji, Elias; Amin, Samir B.; Sharma, Sneha; Williams, Maura; Samant, Neha S.; Dhamdhere, Mayura; Zheng, Norman; Shah, Tara; Shah, Amiksha; Axelrad, Jacob B.; Anvar, Nazanin E.; Lin, Yu-Hsi; Jiang, Shan; Chang, Edward Q.; Ingram, Davis R.; Wang, Wei-Lien; Lazar, Alexander; Lee, Min Gyu; Muller, Florian; Wang, Linghua; Ying, Haoqiang; Rai, Kunal
Cell reports (Cambridge), 10/2020, Letnik: 33, Številka: 3Journal Article
Histone methyltransferase KMT2D harbors frequent loss-of-function somatic point mutations in several tumor types, including melanoma. Here, we identify KMT2D as a potent tumor suppressor in melanoma through an in vivo epigenome-focused pooled RNAi screen and confirm the finding by using a genetically engineered mouse model (GEMM) based on conditional and melanocyte-specific deletion of KMT2D. KMT2D-deficient tumors show substantial reprogramming of key metabolic pathways, including glycolysis. KMT2D deficiency aberrantly upregulates glycolysis enzymes, intermediate metabolites, and glucose consumption rates. Mechanistically, KMT2D loss causes genome-wide reduction of H3K4me1-marked active enhancer chromatin states. Enhancer loss and subsequent repression of IGFBP5 activates IGF1R-AKT to increase glycolysis in KMT2D-deficient cells. Pharmacological inhibition of glycolysis and insulin growth factor (IGF) signaling reduce proliferation and tumorigenesis preferentially in KMT2D-deficient cells. We conclude that KMT2D loss promotes tumorigenesis by facilitating an increased use of the glycolysis pathway for enhanced biomass needs via enhancer reprogramming, thus presenting an opportunity for therapeutic intervention through glycolysis or IGF pathway inhibitors. Display omitted •KMT2D is a tumor suppressor in melanoma•KMT2D rewires metabolic pathways through enhancer reprogramming•KMT2D loss impairs IGFBP5 enhancers and thereby deprives repression to glycolytic genes•KMT2D mutant melanomas are preferentially sensitive to glycolysis and IGFR inhibition Through an in vivo epigenome-focused pooled RNAi screen, Maitituoheti et al. identify KMT2D as a tumor suppressor in melanoma. KMT2D-deficient tumors show substantial reprogramming of key metabolic pathways by reduction of H3K4me1-marked active enhancers, conferring sensitivity to glycolysis and IGFR inhibitors in melanoma with KMT2D-inactivating mutations.
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