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Nasu, Yusuke; Murphy-Royal, Ciaran; Wen, Yurong; Haidey, Jordan N; Molina, Rosana S; Aggarwal, Abhi; Zhang, Shuce; Kamijo, Yuki; Paquet, Marie-Eve; Podgorski, Kaspar; Drobizhev, Mikhail; Bains, Jaideep S; Lemieux, M Joanne; Gordon, Grant R; Campbell, Robert E
Nature communications, 12/2021, Letnik: 12, Številka: 1Journal Article
L-Lactate, traditionally considered a metabolic waste product, is increasingly recognized as an important intercellular energy currency in mammals. To enable investigations of the emerging roles of intercellular shuttling of L-lactate, we now report an intensiometric green fluorescent genetically encoded biosensor for extracellular L-lactate. This biosensor, designated eLACCO1.1, enables cellular resolution imaging of extracellular L-lactate in cultured mammalian cells and brain tissue.
