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van Kampen, Jeroen J A; van de Vijver, David A M C; Fraaij, Pieter L A; Haagmans, Bart L; Lamers, Mart M; Okba, Nisreen; van den Akker, Johannes P C; Endeman, Henrik; Gommers, Diederik A M P J; Cornelissen, Jan J; Hoek, Rogier A S; van der Eerden, Menno M; Hesselink, Dennis A; Metselaar, Herold J; Verbon, Annelies; de Steenwinkel, Jurriaan E M; Aron, Georgina I; van Gorp, Eric C M; van Boheemen, Sander; Voermans, Jolanda C; Boucher, Charles A B; Molenkamp, Richard; Koopmans, Marion P G; Geurtsvankessel, Corine; van der Eijk, Annemiek A
Nature communications, 01/2021, Letnik: 12, Številka: 1Journal Article
Key questions in COVID-19 are the duration and determinants of infectious virus shedding. Here, we report that infectious virus shedding is detected by virus cultures in 23 of the 129 patients (17.8%) hospitalized with COVID-19. The median duration of shedding infectious virus is 8 days post onset of symptoms (IQR 5-11) and drops below 5% after 15.2 days post onset of symptoms (95% confidence interval (CI) 13.4-17.2). Multivariate analyses identify viral loads above 7 log RNA copies/mL (odds ratio OR of 14.7 (CI 3.57-58.1; p < 0.001) as independently associated with isolation of infectious SARS-CoV-2 from the respiratory tract. A serum neutralizing antibody titre of at least 1:20 (OR of 0.01 (CI 0.003-0.08; p < 0.001) is independently associated with non-infectious SARS-CoV-2. We conclude that quantitative viral RNA load assays and serological assays could be used in test-based strategies to discontinue or de-escalate infection prevention and control precautions.
