E-viri
Recenzirano
Odprti dostop
-
Arunachalam, Prabhu S; Scott, Madeleine K D; Hagan, Thomas; Li, Chunfeng; Feng, Yupeng; Wimmers, Florian; Grigoryan, Lilit; Trisal, Meera; Edara, Venkata Viswanadh; Lai, Lilin; Chang, Sarah Esther; Feng, Allan; Dhingra, Shaurya; Shah, Mihir; Lee, Alexandra S; Chinthrajah, Sharon; Sindher, Sayantani B; Mallajosyula, Vamsee; Gao, Fei; Sigal, Natalia; Kowli, Sangeeta; Gupta, Sheena; Pellegrini, Kathryn; Tharp, Gregory; Maysel-Auslender, Sofia; Hamilton, Sydney; Aoued, Hadj; Hrusovsky, Kevin; Roskey, Mark; Bosinger, Steven E; Maecker, Holden T; Boyd, Scott D; Davis, Mark M; Utz, Paul J; Suthar, Mehul S; Khatri, Purvesh; Nadeau, Kari C; Pulendran, Bali
Nature (London), 08/2021, Letnik: 596, Številka: 7872Journal Article
The emergency use authorization of two mRNA vaccines in less than a year from the emergence of SARS-CoV-2 represents a landmark in vaccinology . Yet, how mRNA vaccines stimulate the immune system to elicit protective immune responses is unknown. Here we used a systems vaccinology approach to comprehensively profile the innate and adaptive immune responses of 56 healthy volunteers who were vaccinated with the Pfizer-BioNTech mRNA vaccine (BNT162b2). Vaccination resulted in the robust production of neutralizing antibodies against the wild-type SARS-CoV-2 (derived from 2019-nCOV/USA_WA1/2020) and, to a lesser extent, the B.1.351 strain, as well as significant increases in antigen-specific polyfunctional CD4 and CD8 T cells after the second dose. Booster vaccination stimulated a notably enhanced innate immune response as compared to primary vaccination, evidenced by (1) a greater frequency of CD14 CD16 inflammatory monocytes; (2) a higher concentration of plasma IFNγ; and (3) a transcriptional signature of innate antiviral immunity. Consistent with these observations, our single-cell transcriptomics analysis demonstrated an approximately 100-fold increase in the frequency of a myeloid cell cluster enriched in interferon-response transcription factors and reduced in AP-1 transcription factors, after secondary immunization. Finally, we identified distinct innate pathways associated with CD8 T cell and neutralizing antibody responses, and show that a monocyte-related signature correlates with the neutralizing antibody response against the B.1.351 variant. Collectively, these data provide insights into the immune responses induced by mRNA vaccination and demonstrate its capacity to prime the innate immune system to mount a more potent response after booster immunization.
Avtor
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.