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Krogager, Toke P; Ernst, Russell J; Elliott, Thomas S; Calo, Laura; Beránek, Václav; Ciabatti, Ernesto; Spillantini, Maria Grazia; Tripodi, Marco; Hastings, Michael H; Chin, Jason W
Nature biotechnology, 02/2018, Letnik: 36, Številka: 2Journal Article
We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
