Abstract Objectives Phosphoric acid-Bioglass 45S5 paste can create an interaction layer formed of calcium-phosphate crystals on the dentin surface. In this study, the efficiency of decreasing the dentin permeability exerted by the interaction layer formed between bioglass and dentin was compared to a resin-containing oxalate desensitizing agent (MS Coat One) and a resin-free oxalate desensitizing agent (Super Seal). Methods Dentin permeability was measured before/after a brushing abrasion challenge, followed by examining the top and the fractured dentin surfaces with a field emission scanning electron microscope. Moreover, the chemical nature of the compounds formed on top of the dentin surface was examined using the field emission scanning electron microscope (FE-SEM) equipped with an energy-dispersive X-ray spectroscope (EDS), and the crystalline structures of the dentinal surfaces were examined by X-ray diffraction (XRD). Results The results showed that application of 45S5 bioglass paste to dentin was able to occlude patent dentinal tubule orifices with a layer of calcium-phosphate crystals, while the oxalate containing agents were able to form small crystals which were found in dentinal tubule orifices and scattered along the superficial parts of the dentinal tubule lumen. The brushing-abrasion challenge significantly increased the permeability of dentin treated by Super Seal and MS Coat One, while these challenges had no significant effect on the dentin permeability of specimens treated with 45S5 bioglass paste. Significance The new technique provided better durability than two products available on the market. Moreover, our previous research showed the biocompatibility of using this technique on dental pulp cells, suggesting that this technique can aid in treating dentin hypersensitivity cases.