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Mukherjee, Sanjukta; Murata, Asako; Ishida, Ryoga; Sugai, Ayako; Dohno, Chikara; Hamada, Michiaki; Krishna, Sudhir; Nakatani, Kazuhiko
Molecular therapy. Nucleic acids, 03/2022, Letnik: 27Journal Article
Selective targeting of biologically relevant RNAs with small molecules is a long-standing challenge due to the lack of clear understanding of the binding RNA motifs for small molecules. The standard SELEX procedure allows the identification of specific RNA binders (aptamers) for the target of interest. However, more effort is needed to identify and characterize the sequence-structure motifs in the aptamers important for binding to the target. Herein, we described a strategy integrating high-throughput (HT) sequencing with conventional SELEX followed by bioinformatic analysis to identify aptamers with high binding affinity and target specificity to unravel the sequence-structure motifs of pre-miRNA, which is essential for binding to the recently developed new water-soluble small-molecule CMBL3aL. To confirm the fidelity of this approach, we investigated the binding of CMBL3aL to the identified motifs by surface plasmon resonance (SPR) spectroscopy and its potential regulatory activity on dicer-mediated cleavage of the obtained aptamers and endogenous pre-miRNAs comprising the identified motif in its hairpin loop. This new approach would significantly accelerate the identification process of binding sequence-structure motifs of pre-miRNA for the compound of interest and would contribute to increase the spectrum of biomedical application. Display omitted Using HT-SELEX, a binding sequence-structure motif in pre-miRNA for the small molecule CMBL3aL was identified. The fidelity of this approach was validated by SPR-based binding assays and in vitro dicer cleavage reactions of CMBL3aL with enriched RNA aptamer sequences. We further evaluated the potential applicability using endogenous pre-miRNAs comprising the characteristic binding sequence-structure motif for CMBL3aL.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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