Abstract One of the main challenges in the boundary-layer turbulence research consists in advancing turbulence theory towards describing turbulent regimes over inhomogeneous and irregular surface which is often the case in nature. Novel observational techniques are required to sample turbulence statistics in such conditions. To that aim, a light-weight payload for atmospheric turbulence measurements is designed and used on a vertical take-off and landing (VTOL) fixed-wing unmanned aerial vehicle (UAV). Such an observational platform complements traditional mast observations and makes it possible to sample turbulence statistics over remote and inhomogeneous landscapes. The advantages of the VTOL scheme consist in: i) minimizing the risk of the payload damage during landing; ii) less strict requirements to the surface conditions in the landing area. The turbulence payload consists of a miniature seven-hole probe, inertial navigation system and a fine-wire resistance thermometer. The seven-hole probe and the fine-wire temperature sensor were designed and calibrated as part of this study. In addition, the payload also includes a slow-response temperature-humidity sensor, relatively fast humidity sensor as well as an infrared surface temperature sensor. For the onboard data-logging, a computer based on Raspberry Pi 4 is used. The whole system has been successfully tested at the Tsimlyansk research station, Russia.