Summary Background Approximately 40% of chronic idiopathic urticaria (CIU) subjects have autoantibodies to either FcɛRIα or IgE. The effect of such autoantibodies on circulating basophil activation status is unknown. Objective The expression of cell surface activation markers on basophils from CIU, non‐allergic, and allergic subjects were compared. Further, the relationship between marker expression and serum factors reported in CIU, such as histamine‐releasing activity (HRA) and immunoreactivity to FcɛRIα were examined. Methods Peripheral blood was obtained from CIU, allergic, and non‐allergic donors and fractionated by density gradients. Enriched basophils (1–12%) were analysed by flow cytometry for expression of activation markers including CD63, CD69, and CD203c. Dilutions of serum (5–50%) were analysed for HRA on basophils from a normal donor. Serum was tested for immunoreactivity by western blotting to a standard cell lysate prepared from an RBL‐SX38 cell line transfected with human FcɛRIα. Results CIU subjects (n=9) and allergic subjects (n=8) exhibited enhanced expression of CD63 and CD69, as compared with non‐allergic subjects (n=7); however, no difference was seen among groups for CD203c expression. Five CIU and two non‐allergic subjects had evidence of significant serum HRA (>20%), whereas two CIU, two allergic, and three non‐allergic subjects had evidence of serum immunoreactivity to FcɛRIα. Serum HRA and serum immunoreactivity to FcɛRIα were not associated with enhanced surface marker expression. Conclusion Basophil activation marker expression is increased in CIU subjects and is not associated with serum factors. In addition, serum HRA and FcɛRIα immunoreactivity are not unique to CIU, or related to enhanced circulating basophil marker expression.