We describe a novel glucose biosensor based on a nanoband array electrode design, manufactured using standard semiconductor processing techniques, and bio-modified with glucose oxidase immobilized at the nanoband electrode surface. The nanoband array architecture allows for efficient diffusion of glucose and oxygen to the electrode, resulting in a thousand-fold improvement in sensitivity and wide linear range compared to a conventional electrode. The electrode constitutes a robust and manufacturable sensing platform. •Glucose oxidase was covalently attached to thiol-modified nanoband array electrodes.•The response of the sensor to hydrogen peroxide and glucose was monitored electrochemically.•Comparing with a conventional electrode, a thousand-fold improvement in sensitivity and wide linear range was obtained.•The superior performance of the nanoband sensor can be attributed to the nanoband array architecture.•The nanoband structure allows for efficient diffusion of substrate to the electrode and provides low background currents.