The abscisic acid receptor PYL8 plays an important role for regulation of root abscisic acid sensitivity, and abscisic acid-dependent inhibition of PP2Cs by PYR/PYLs is required for root hydrotropism. Abscisic acid ( ABA ) signaling plays a critical role in regulating root growth and root system architecture. ABA -mediated growth promotion and root tropic response under water stress are key responses for plant survival under limiting water conditions. In this work, we have explored the role of Arabidopsis ( Arabidopsis thaliana ) PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS for root ABA signaling. As a result, we discovered that PYL8 plays a nonredundant role for the regulation of root ABA sensitivity. Unexpectedly, given the multigenic nature and partial functional redundancy observed in the PYR/PYL family, the single pyl8 mutant showed reduced sensitivity to ABA -mediated root growth inhibition. This effect was due to the lack of PYL8-mediated inhibition of several clade A phosphatases type 2C ( PP2Cs ), since PYL8 interacted in vivo with at least five PP2Cs , namely HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABA-INSENSITIVE1 (ABI1), ABI2, and PP2CA/ABA-HYPERSENSITIVE GERMINATION3 as revealed by tandem affinity purification and mass spectrometry proteomic approaches. We also discovered that PYR/PYL receptors and clade A PP2Cs are crucial for the hydrotropic response that takes place to guide root growth far from regions with low water potential. Thus, an ABA -hypersensitive pp2c quadruple mutant showed enhanced hydrotropism, whereas an ABA -insensitive sextuple pyr/pyl mutant showed reduced hydrotropic response, indicating that ABA -dependent inhibition of PP2Cs by PYR/PYLs is required for the proper perception of a moisture gradient.