The aim of the study was to determine how the values of ambient temperature affect the welfare of the animals with regard to the average performance. The study was carried out in the agricultural company Petrovice during the summer and winter seasons. The experiment included Holstein dairy cattle. Air temperature was measured using a data logger with sensors (Datalogger COMET 3120) in the stable. Data on average yield were taken from farm records and then processed using Microsoft Excel. The air temperature is very variable and its changes animals react immediately. Measured values of air temperature in the stable are important for optimal welfare. It affects the productivity of dairy cows, milk quality, reproduction and animal health.