Provider: - Institution: - Data provided by Europeana Collections- Actinomycetes are Gram-positive soil bacteria known as producers of secondary bioactive metabolites. In the search for new bioactive compounds collection of actinomycetes from the Laboratory for Microbial Molecular Genetics and Ecology (Institute of Molecular Genetic and Genetic Engineering, University of Belgrade) was screened according to several criteria - bioactivity, the presence of the genes for polyketide synthase (PKS), pigmentation, and crude culture extracts absorption profile in ultraviolet and visible (UV/Vis) spectra. Based on these we have isolated three strains NP10, JS520, and Streptomyces durmitotensis, previously renewed for didehydroroflamicoin (DDHR) production. Isolates NP10 and JS520 were identified to belong to the genus Streptomyces. Streptomyces sp. NP10 accumulated branched chain fatty acids, predominately isopalmitic, and cyclic dipeptides consisting of proline in combination with valine, isoleucine or alanine, that have a stimulating effect on the trophoblast cell line in culture at low concentrations (1 ng/ml and 1 mg/ml) and cytotoxic effect at high concentrations (1 mg/ml). Streptomyces sp. JS520 was determined to be exceptionally good producer of pigment undecylprodigiosine (UP) with antibacterial, antifungal, antioxidant and UV protective activities showing cytotoxic effects on trophoblast cell line in culture at high concentrations (1 ng/ml). Cytotoxicity of polyene macrolide antibiotic DDHR from S. durmitotensis was confirmed and it’s antifungal effects were shown. PKS cluster responsible for DDHR synthesis was subcloned in the cosmid library and partially sequenced. Optimization of culture conditions resulted in 2.12 times increased production of UP, and 1.1 times increased production of DDHR.- Aktinomicete su Gram-pozitivne zemljišne bakterije poznate kao proizvođači bioaktivnih sekundarnih metabolita. U cilju pronalaženja novih bioaktivnih jedinjenja pretraživana je kolekcija aktinomiceta Laboratorije za molekularnu gentiku i ekologiju mikroorganizama Instituta za molekularnu gentiku i genetičko inženjerstvo Univerziteta u Beogradu po više kriterijuma – bioaktivnosti, prisustvu gena za poliketid sintaze (PKS), pigmentaciji i profilu apsorpcije ukupnih ekstrakata kultura u ultraljubičastom i vidljivom (UV/Vis) delu spektra. Na osnovu ovoga izdvojena su tri izolata NP10, JS520 i Streptomyces durmitorensis, od ranije poznati proizvođač didehidroroflamikoina (DDHR). Izolati NP10 i JS520 identifikovani su kao pripadnici roda Streptomyces. Streptomyces sp. NP10 akumulirao je masne kiseline račvastog niza, pre svega izopalmitinsku kiselinu, i ciklične dipeptide sastavljene od prolina u kombinaciji sa valinom, izoleucinom ili alaninom koji su imali stimulativno dejstvo na trofoblastne ćelije u kulturi u niskim koncentracijama (1 ng/ml i 1 μg/ml) i citotoksično dejstvo u visokim (1 mg/ml) koncentracijama. Pokazano je da Streptomyces sp. JS520 ima sposobnost proizvodnje velike količine pigmenta undecilprodigiozina (UP) sa antibakterijskim, antimikotičkim, antioksidativnim i UV protektivnim osobinama koji je imao citotoksično dejstvo na trofoblastne ćelije u kulturi u visokim koncentracijama (1 ng/ml). Citotoksičnost polienskog makrolidnog antibiotika DDHR iz S. durmitorensis je potvrđena, a pokazan je i njegov antimikotički efekat. PKS klaster zadužen za sintezu DDHR je subkloniran u kozmidnu biblioteku i delimično sekvenciran. Optimizacijom uslova gajenja količina proizvedenog UP povećana je 2,12 puta, a DDHR 1,1 puta.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana