Objective: To evaluate the genotoxic effect on workers exposed to X-rays in the Radiology Service of the Luis N. Sáenz National Hospital PNP. Materials and methods. The type of study was observational, prospective, cross-sectional, analytical, using the comet assay as an analysis technique. The study population was 20 workers exposed to X-rays and 20 people without exposure. Results The mean length of migration of damaged DNA in the control group was 1.28 ± 0.38 µm and 10.39 ± 9.44 µm for the exposed group, the means of the groups were compared, obtaining p = 0.001 significant. The correlation analysis for DNA damage, years of exposure and dose received, a significant correlation was found (p <0.05). For the correlation of DNA damage with age, no statistical significance was found (p> 0.05). Conclusions X-rays at low permissible doses can cause damage to DNA integrity, correlating with the first years of exposure of personnel working in the radiology service. Keywords: Genotoxic, exposure, X-ray, comet test.