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Osamitev, identifikacija in karakterizacija gliv iz avtomehanične delavnice : magistrsko delo : magistrski študij - 2. stopnja = Isolation, identification and characterisation of fungi from car service shop : M. Sc. Thesis : Master Study Programmes
Gnidovec, Tim
Vse večje število avtomobilov in drugih motornih vozil povečuje onesnaženost ozračja, talne, gozdne in vodne ekosisteme z ogljikovodiki. Z namenom najti glivne seve, ki bi jih lahko uporabili v ...bioremediacijskih postopkih, smo preverili njihovo prisotnost v avtomobilih, in sicer v delih, ki pridejo v stik z bencinom, oljem ali nafto. Vzorčili smo čistilce olja, goriva, ter odprtino za dovod goriva. Poleg tega smo v avtomehanični delavnici vzorčili odtočni jašek interne kanalizacije in talne oljne madeže. Iz vzorcev sem s pomočjo različnih izolacijskih tehnik osamil 45 izolatov gliv. Identificirali smo jih s pomočjo nukleotidih zaporedij izbranih molekularnih označevalcev za posamezen rod. Seve smo uvrstili v 13 rodov, 11 sevov v deblo Ascomycota (Acremonium, Alternaria, Aureobasidium, Candida, Cladosporium, Coniochaeta, Exophiala, Geotrichum, Meyerozyma, Penicilium, Pleurostoma), 2 seva v deblo Basidiomycota (Cystobasidium in Rhodotorula). Več kot polovica izolatov (26) je pripadala rodovom Aureobasidium, Candida, Meyerozyma in Rhodotorula. Vse izolirane glive smo testirali na zmožnost razgradnje toluena, mineralnega olja in n-heksadekana. Vsi izolati so rastli na toluenu, medtem ko je bila rast na n-heksadekanu in mineralnem olju omejena na manj kot 50%, in sicer na rodove Meyerozyma, Acremonium, Penicilium, Cladosporum in Exophiala.
Type of material - master's thesis
; adult, serious
Publication and manufacture - Ljubljana : [T. Gnidovec], 2022
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