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Elucidation of the AI-2 communication system in the food-borne pathogen Campylobacter jejuni by whole-cell-based biosensor quantificationJug, Dina ...The food-borne pathogen Campylobacter jejuni produces autoinducer-2 (AI-2) as an interspecies signalling molecule. AI-2 can trigger enhanced colonisation and biofilm formation, and this poses a ... serious risk to public health. To date, this communication system of C. jejuni is only partially understood, as detection and quantifi- cation of such autoinducer signalling molecules in complex media is hard to achieve. We have developed a whole-cell Vibrio harveyi-based biosensor assay to accurately quantify and follow production of AI-2 by C. jejuni 81–176 in a defined growth medium and in a model food system. Several V. harveyi strains were tested, but the most sensitive bioluminescent response to C. jejuni AI-2 was achieved with V. harveyi MM30, likely due to its ability to self-amplify the response to AI-2. The AI-2 concentrations measured by this biosensor were confirmed using an independent analytical method, HPLC-FLD, which we introduced for Campylobacter analytics for the first time. The AI-2 concentration produced by C. jejuni 81–176 in the model food system was ~5-fold that in the defined growth medium, at the same cell density. Together with the linear increments in AI-2 concentrations with cell density, this suggests that in C. jejuni, AI-2 represents a metabolic by-product rather than a true quorum- sensing molecule. This biosensor method is highly sensitive, as shown by the reduction in the limit of detection (by a factor of 100) compared to HPLC-FLD, and it enables quantification of AI-2 in complex matrices, such as food, which will help to improve the quality and safety of food production.Source: Biosensors & bioelectronics. - ISSN 0956-5663 (Vol. 212, 2022, str. 1-8)Type of material - article, component part ; adult, seriousPublish date - 2022Language - englishCOBISS.SI-ID - 110249219
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Jug, Dina | 51861 |
Klančnik, Anja | 22491 |
Smole Možina, Sonja | 07030 |
Dogša, Iztok | 24407 |
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