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Art v 1 IgE epitopes of patients and humanized mice are conformational [Elektronski vir]Zabel, Maja ...Background. Worldwide, pollen of the weed mugwort (Artemisia vulgaris) is a major cause of severe respiratory allergy, with its major allergen, Art v 1, being the key pathogenic molecule for millions ... of patients. Humanized mice transgenic for a human T-cell receptor specific for the major Art v 1 T-cell epitope and the corresponding HLA have been made. Objective. We sought to characterize IgE epitopes of Art v 1–sensitized patients and humanized mice for molecular immunotherapy of mugwort allergy. Methods. Four overlapping peptides incorporating surface-exposed amino acids representing the full-length Art v 1 sequence were synthesized and used to search for IgE reactivity to sequential epitopes. For indirect mapping, peptide-specific rabbit antibodies were raised to block IgE against surface-exposed epitopes on folded Art v 1. IgE reactivity and basophil activation studies were performed in clinically defined mugwort-allergic patients. Secondary structure of recombinant (r) Art v 1 and peptides was determined by circular dichroism spectroscopy. Results. Mugwort-allergic patients and humanized mice sensitized by allergen inhalation showed IgE reactivity and/or basophil activation mainly to folded, complete Art v 1 but not to unfolded, sequential peptide epitopes. Blocking of allergic patients’ IgE with peptide-specific rabbit antisera identified a hitherto unknown major conformational IgE binding site in the C-terminal Art v 1 domain. Conclusions. Identification of the new major conformational IgE binding site on Art v 1, which can be blocked with IgG raised against non-IgE reactive Art v 1 peptides, is an important basis for the development of a hypoallergenic peptide vaccine for mugwort allergy.Source: The journal of allergy and clinical immunology [Elektronski vir]. - ISSN 1097-6825 (Vol. 150, iss. 4, Oct. 2022, str. 920-930)Type of material - e-article ; adult, seriousPublish date - 2022Language - englishCOBISS.SI-ID - 116269571
Author
Zabel, Maja |
Weber, Milena |
Kratzer, Bernhard |
Köhler, Cordula |
Jahn-Schmid, Beatrice |
Gadermaier, Gabriele |
Gattinger, Pia |
Bidovec, Urška |
Korošec, Peter, 1970- |
Smole, Ursula |
Valenta, Rudolf, 1963- |
Pickl, Winfried F.
Topics
Allergy and immunology |
Hypersensitivity |
Allergens |
Epitope mapping |
Artemisia |
Desensitizaion, immunologic |
Preobčutljivost |
Alergeni |
Alergija in imunologija |
Kartiranje epitopov |
Artemisia |
Imunološka desenzibilizacija |
alergija za pelod pelina |
epitop IgE |
specifična imunoterapija |
mugwort pollen allergy |
IgE epitope |
allergen-specific immunotherapy
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Zabel, Maja | ![]() |
Weber, Milena | ![]() |
Kratzer, Bernhard | ![]() |
Köhler, Cordula | ![]() |
Jahn-Schmid, Beatrice | ![]() |
Gadermaier, Gabriele | ![]() |
Gattinger, Pia | ![]() |
Bidovec, Urška | 25169 |
Korošec, Peter, 1970- | 22807 |
Smole, Ursula | ![]() |
Valenta, Rudolf, 1963- | ![]() |
Pickl, Winfried F. | ![]() |
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