-
Art v 1 IgE epitopes of patients and humanized mice are conformational [Elektronski vir]Zabel, Maja ...Background. Worldwide, pollen of the weed mugwort (Artemisia vulgaris) is a major cause of severe respiratory allergy, with its major allergen, Art v 1, being the key pathogenic molecule for millions ... of patients. Humanized mice transgenic for a human T-cell receptor specific for the major Art v 1 T-cell epitope and the corresponding HLA have been made. Objective. We sought to characterize IgE epitopes of Art v 1–sensitized patients and humanized mice for molecular immunotherapy of mugwort allergy. Methods. Four overlapping peptides incorporating surface-exposed amino acids representing the full-length Art v 1 sequence were synthesized and used to search for IgE reactivity to sequential epitopes. For indirect mapping, peptide-specific rabbit antibodies were raised to block IgE against surface-exposed epitopes on folded Art v 1. IgE reactivity and basophil activation studies were performed in clinically defined mugwort-allergic patients. Secondary structure of recombinant (r) Art v 1 and peptides was determined by circular dichroism spectroscopy. Results. Mugwort-allergic patients and humanized mice sensitized by allergen inhalation showed IgE reactivity and/or basophil activation mainly to folded, complete Art v 1 but not to unfolded, sequential peptide epitopes. Blocking of allergic patients’ IgE with peptide-specific rabbit antisera identified a hitherto unknown major conformational IgE binding site in the C-terminal Art v 1 domain. Conclusions. Identification of the new major conformational IgE binding site on Art v 1, which can be blocked with IgG raised against non-IgE reactive Art v 1 peptides, is an important basis for the development of a hypoallergenic peptide vaccine for mugwort allergy.Source: The journal of allergy and clinical immunology [Elektronski vir]. - ISSN 1097-6825 (Vol. 150, iss. 4, Oct. 2022, str. 920-930)Type of material - e-article ; adult, seriousPublish date - 2022Language - englishCOBISS.SI-ID - 116269571
Author
Zabel, Maja |
Weber, Milena |
Kratzer, Bernhard |
Köhler, Cordula |
Jahn-Schmid, Beatrice |
Gadermaier, Gabriele |
Gattinger, Pia |
Bidovec, Urška |
Korošec, Peter, 1970- |
Smole, Ursula |
Valenta, Rudolf, 1963- |
Pickl, Winfried F.
Topics
Allergy and immunology |
Hypersensitivity |
Allergens |
Epitope mapping |
Artemisia |
Desensitizaion, immunologic |
Preobčutljivost |
Alergeni |
Alergija in imunologija |
Kartiranje epitopov |
Artemisia |
Imunološka desenzibilizacija |
alergija za pelod pelina |
epitop IgE |
specifična imunoterapija |
mugwort pollen allergy |
IgE epitope |
allergen-specific immunotherapy
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Zabel, Maja | |
Weber, Milena | |
Kratzer, Bernhard | |
Köhler, Cordula | |
Jahn-Schmid, Beatrice | |
Gadermaier, Gabriele | |
Gattinger, Pia | |
Bidovec, Urška | 25169 |
Korošec, Peter, 1970- | 22807 |
Smole, Ursula | |
Valenta, Rudolf, 1963- | |
Pickl, Winfried F. |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.