ALL libraries (COBIB.SI union bibliographic/catalogue database)
PDF
  • Engineering a GPCR−ligand pair that simulates the activation of d2l by dopamine [Elektronski vir]
    Tschammer, Nuška ...
    In the past decade, engineered G-protein-coupled receptors activated solely by synthetic ligands (RASSLs) have been implemented as a new means to study neurotransmission, which is controlled by ... G-protein-coupled receptors in vitro and in vivo. In this study, we report an engineered dopamine receptor D2L F3906.52W, which is the first identified RASSL for the dopamine receptor family. The mutant receptor is characterized by a disrupted ligand binding and complete loss of efficacy for the endogenous ligand, dopamine, which is putatively due to a sterically induced perturbation of H-bonding with conserved serine residues in TM5. Based on this model, we rationally developed an aminoindanederived set of agonists. Because these agonists forgo analogous H-bonding functionalities, their binding energy does not depend on the respective interactions. Binding affinity and potency were optimized by ligand modifications bearing molecular appendages that obviously interact with a secondary recognition site provided by four hydrophobic residues in TM2 and TM3. Thus, the ferrocenyl carboxamide 5b (FAUC 185) was identified as a synthetic agonist that is able to stimulate the mutant receptor in a manner similar to that by which endogenous dopamine activates the D2L wild-type receptor. The engineered dopamine receptor D2L F3906.52W in combination with FAUC 185 (5b) provides a new tool to probe GPCR functions selectively in specific cell populations in vitro and in vivo.
    Source: ACS chemical neuroscience. - ISSN 1948-7193 (Vol. 1, iss. 1, 2010, str. 25-35)
    Type of material - e-article
    Publish date - 2010
    Language - english
    COBISS.SI-ID - 121179907

    Link(s):

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368624/pdf/cn900001b.pdf
    DOI

Shelf entry