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FAM19A4/miR124-2 methylation testing and human papillomavirus (HPV) 16/18 genotyping in HPV-positive women under the age of 30 yearsVink, Frederique J. ...Background High-grade squamous intraepithelial lesions (HSIL) or cervical intraepithelial neoplasia (CIN) grade 2/3 lesions in human papillomavirus (HPV)–positive women <30 years of age have high ... spontaneous regression rates. To reduce overtreatment, biomarkers are needed to delineate advanced CIN lesions that require treatment. We analyzed the FAM19A4/miR124-2 methylation test and HPV16/18 genotyping in HPV-positive women aged <30 years, aiming to identify CIN2/3 lesions in need of treatment. Methods A European multicenter retrospective study was designed evaluating the FAM19A4/miR124-2 methylation test and HPV16/18 genotyping in cervical scrapes of 1061 HPV-positive women aged 15–29 years (690 ≤CIN1, 166 CIN2, and 205 CIN3+). A subset of 62 CIN2 and 103 CIN3 were immunohistochemically characterized by HPV E4 expression, a marker for a productive HPV infection, and p16ink4a and Ki-67, markers indicative for a transforming infection. CIN2/3 lesions with low HPV E4 expression and high p16ink4a/Ki-67 expression were considered as nonproductive, transforming CIN, compatible with advanced CIN2/3 lesions in need of treatment. Results FAM19A4/miR124-2 methylation positivity increased significantly with CIN grade and age groups (<25, 25–29, and ≥30 years), while HPV16/18 positivity was comparable across age groups. FAM19A4/miR124-2 methylation positivity was HPV type independent. Methylation-positive CIN2/3 lesions had higher p16ink4a/Ki-67-immunoscores (P = .003) and expressed less HPV E4 (P = .033) compared with methylation-negative CIN2/3 lesions. These differences in HPV E4 and p16ink4a/Ki-67 expression were not found between HPV16/18–positive and non-16/18 HPV–positive lesions. Conclusions Compared with HPV16/18 genotyping, the FAM19A4/miR124-2 methylation test detects nonproductive, transforming CIN2/3 lesions with high specificity in women aged <30 years, providing clinicians supportive information about the need for treatment of CIN2/3 in young HPV-positive women.Source: Clinical infectious diseases. - ISSN 1058-4838 (Vol. 76, iss. 3, Feb. 2023, str. e827-e834)Type of material - article, component partPublish date - 2023Language - englishCOBISS.SI-ID - 129633283
Author
Vink, Frederique J. |
Meijer, Chris J.L.M. |
Hesselink, Albertus |
Floore, Arno N. |
Lissenberg-Witte, Birgit I. |
Bonde, Jesper |
Pedersen, Helle |
Cuschieri, Kate S. |
Poljak, Mario, 1965- |
Oštrbenk Valenčak, Anja
Topics
rak materničnega vratu |
cervikalna intraepitelna neoplazija |
humani papiloma virus |
cervical cancer |
cervical intraepithelial neoplasia |
human papillomavirus
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Vink, Frederique J. | ![]() |
Meijer, Chris J.L.M. | ![]() |
Hesselink, Albertus | ![]() |
Floore, Arno N. | ![]() |
Lissenberg-Witte, Birgit I. | ![]() |
Bonde, Jesper | ![]() |
Pedersen, Helle | ![]() |
Cuschieri, Kate S. | ![]() |
Poljak, Mario, 1965- | 11622 |
Oštrbenk Valenčak, Anja | 35359 |
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