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Analysis of galactoglucomannans from spruce wood by capillary electrophoresisDoliška, Aleš ...The aim of this study was to setup a method for detection and quantification of monosaccharide components in technical galactoglucomannas (T-GGM) from spruce wood using capillary zone electrophoresis ... (CZE). CZE technique was optimised regarding borate buffer concentrations, EOF modifier application, and system pH. Aqueous solution of T-GGM was chemically hydrolysed by sulphuric acid, in an autoclave. In this way obtained monosaccharides were derivatized with 4-amino benzoic acid ethyl ester via reductive amination using sodium cyanoborohydride. The results of the optimisation procedure showed that the borate buffers at lowest concentrations (100 and 200 mM) with acetonitrile addition as EOF modifier gave the optimal measurement results, as it showed sufficient separation at relatively short migration times. The amounts of single monosaccharide components in the T-GGM samples obtained by the optimised CZE procedure were practically the same in comparison to the results of the well established HPLC-anion exchange chromatography. On the basis of this research, it was concluded that the capillary zone electrophoresis is an efficient analytical procedure for the characterisation of galactoglucomannans derived from softwoods.Source: Cellulose. - ISSN 0969-0239 (Issue 6, Vol. 16, 2009, str. 1089-1097)Type of material - article, component partPublish date - 2009Language - englishCOBISS.SI-ID - 13313046
Author
Doliška, Aleš |
Strnad, Simona, 1965- |
Ribitsch, Volker |
Stana-Kleinschek, Karin |
Willför, Stefan |
Saake, Bodo
Topics
kapilarna elektroforeza |
ogljikovi hidrati |
galaktoglukomanan |
glukomanan |
polisaharidi |
capillary electrophoresis |
carbohydrates |
galactoglucomannan |
glucomannan |
polysaccharide
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Doliška, Aleš | 17577 |
Strnad, Simona, 1965- | 10575 |
Ribitsch, Volker | 15077 |
Stana-Kleinschek, Karin | 07814 |
Willför, Stefan | |
Saake, Bodo |
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