-
Integrative transcriptomic analysis in human and mouse model of anaphylaxis identifies gene signatures associated with cell movement, migration and neuroinflammatory signalling [Elektronski vir]Rijavec, Matija ...Background: Anaphylaxis is an acute life-threatening allergic reaction and a concern at a global level; therefore, further progress in understanding the underlying mechanisms and more effective ... strategies for diagnosis, prevention and management are needed. Objective: We sought to identify the global architecture of blood transcriptomic features of anaphylaxis by integrating expression data from human patients and mouse model of anaphylaxis. Methods: Bulk RNA-sequencings of peripheral whole blood were performed in: i) 14 emergency department (ED) patients with acute anaphylaxis, predominantly to Hymenoptera venom, ii) 11 patients with peanut allergy undergoing double-blind, placebo-controlled food challenge (DBPCFC) to peanut, iii) murine model of IgE-mediated anaphylaxis. Integrative characterisation of differential gene expression, immune cell-type-specific gene expression profiles, and functional and pathway analysis was undertaken. Results: 1023 genes were commonly and significantly dysregulated during anaphylaxis in ED and DBPCFC patients; of those genes, 29 were also dysregulated in the mouse model. Cell-type-specific gene expression profiles showed a rapid downregulation of blood basophil and upregulation of neutrophil signature in ED and DBPCFC patients and the mouse model, but no consistent and/or significant differences were found for other blood cells. Functional and pathway analysis demonstrated that human and mouse blood transcriptomic signatures of anaphylaxis follow trajectories of upregulation of cell movement, migration and neuroinflammatory signalling, and downregulation of lipid activating nuclear receptors signalling. Conclusion: Our study highlights the matched and extensive blood transcriptomic changes and suggests the involvement of discrete cellular components and upregulation of migration and neuroinflammatory pathways during anaphylaxis.Source: Frontiers in immunology. - ISSN 1664-3224 (Vol. 13, 2022, str. 1-15)Type of material - e-article ; adult, seriousPublish date - 2022Language - englishCOBISS.SI-ID - 133240067
Author
Rijavec, Matija |
Maver, Aleš, 1984- |
Turner, Paul J. |
Hočevar, Keli |
Košnik, Mitja |
Yamani, Amnah |
Hogan, Simon P. |
Custovic, Adnan |
Peterlin, Borut, 1963- |
Korošec, Peter, 1970-
Topics
Anaphylaxis |
Diagnosis |
Mast cells |
Basophils |
Neutrophils |
Anafilaksija |
Diagnostika |
Mastociti |
Bazofilci |
Nevtrofilci |
gibanje celic |
migracija |
transcriptome analysis |
cell movement |
migration |
neuroinflammatory signaling |
lipid activating nuclear receptors signaling
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Rijavec, Matija | 29300 |
Maver, Aleš, 1984- | 34579 |
Turner, Paul J. | ![]() |
Hočevar, Keli | 38272 |
Košnik, Mitja | 10921 |
Yamani, Amnah | ![]() |
Hogan, Simon P. | ![]() |
Custovic, Adnan | ![]() |
Peterlin, Borut, 1963- | 10458 |
Korošec, Peter, 1970- | 22807 |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.