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Microtiterplate phosphate assay based on luminescence quenching of a terbium complex amenable to decay time detection
Turel, Matejka ...
We describe a terbium-ligand complex (TbL) for a microtiterplate assay for phosphate (P) in the 0.3-100 mol L-1 range based on luminescence quenching. As the pH optimum is at neutral pH (7.4) the ...probe is quenched by both, primary (H2PO4-) and secondary phosphate (HPO42-). The LOD is 110 nmol L-1. A Stern-Volmer study revealed that quenching is mostly static. Due to the ms-decay time of TbL, the first luminescence lifetime assay for phosphate could also be developed. The lifetime-based calibration plot is linear between0.5 and 5 mol L-1 of P. The effect of various surfactants on assay performance and a study on interferents are presented. The probe was successfully applied to determination of P in commercial plant fertilizers and validated against the molybdenum blue test. The probe is the most sensitive lanthanide-based probe for phosphate.
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