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Zirconia-toughened alumina ceramic wear particles do not elicit inflammatory responses in human macrophages [Elektronski vir]Porporati, Alessandro Alan ...Ten percent of patients undergoing total hip arthroplasty (THA) require revision surgery. One of the reasons for THA are wear particles released from the implants that can activate the immune defense ... and cause osteolysis and failure of the joint implant. The discrepancies between reports on toxicity and immunogenicity of the implant materials led us to this study in which we compared toxicity and immunogenicity of well-defined nanoparticles from Al2O3, zirconia-toughened alumina (ZTA), and cobalt chrome (CoCr), a human THP-1 macrophage cell line, human PBMCs, and therefrom-derived primary macrophages. None of the tested materials decreased the viability of THP-1 macrophages nor human primary macrophages at the 24 h time point, indicating that at concentrations from 0.05 to 50 µm3/cell the tested materials are non-toxic. Forty-eight hours of treatment of THP-1 macrophages with 5 µm3/cell of CoCr and Al2O3 caused 8.3-fold and 4.6-fold increases in TNF-α excretion, respectively, which was not observed for ZTA. The comparison between THP-1 macrophages and human primary macrophages revealed that THP-1 macrophages show higher activation of cytokine expression in the presence of CoCr and Al2O3 particles than primary macrophages. Our results indicate that ZTA is a non-toxic implant material with no immunogenic effects in vitro.Source: International journal of molecular sciences [Elektronski vir]. - ISSN 1422-0067 (Vol. 24, art. 6482, iss. 7, 2023, 12 str.)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 147226627
Author
Porporati, Alessandro Alan |
Mödinger, Yvonne |
Fischer, Sarah |
Polajžer, Sara |
Mettang, Melanie |
Deisinger, Ulrike |
Podlogar, Matejka |
Trebše, Rihard |
Lovšin, Nika
Topics
Citokini |
Nanomateriali |
Vnetje |
orthopaedic implant |
alumina |
zirconia-toughened alumina (ZTA) |
nanoparticles |
macrophages |
cell viability |
inflammation |
cytokine secretion |
ortopedski vsadek |
aluminijev oksid |
aluminijev oksid utrjen s cirkonijem |
nanodelci |
makrofagi |
sposobnost preživetja celic |
vnetje |
izločanje citokinov
Author | Porporati, Alessandro Alan ... |
Title | Zirconia-toughened alumina ceramic wear particles do not elicit inflammatory responses in human macrophages [Elektronski vir] |
Publication date | 2023-03-30 |
COBISS.SI-ID | 147226627 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
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Mezenhimske matične celice-nosilci endogene regenerativne sposobnosti tkiv v boju proti staranju mišično-skeletnega sistema | J3-1749-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
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https://www.mdpi.com/1422-0067/24/7/6482 |
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=145026 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Porporati, Alessandro Alan | |
Mödinger, Yvonne | |
Fischer, Sarah | |
Polajžer, Sara | |
Mettang, Melanie | |
Deisinger, Ulrike | |
Podlogar, Matejka | 27843 |
Trebše, Rihard | 23524 |
Lovšin, Nika | 19649 |
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