Opazovanje razvoja dogodkov v živih celicah je ključnega pomena za razvoj medicine in biologije, zato ta članek opisuje zajem takih posnetkov v prostoru in času oz. v 4D. Ker slikanje pogosto temelji ...na uporabi fluorescenčnih mikroskopov, se članek v prvem delu osredotoča na pojav fluorescence in osnove mikroskopije, v drugem delu pa na dve novi metodi fluorescenčne mikroskopije, ki omogočata hitro in natančno slikanje celotne debeline vzorca.
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