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Coexpression of myosin heavy chain 2b with myosin heavy chain 1 - fact or artefact?Eržen, Ida ...In skeletal muscle, pure fibres expressing one myosin heavy chain (MyHC) isoform, and intermediate fibres, expressing two and exceptionally three MyHCshave been described. When skeletal muscle adapts ... its fibre type profile to changed functional demands MyHC isoform transformation follows the pathway:MyHC-1 - MyHC-2a - MyHC-2x/d - MyHC-2b. Therefore, in hybrid fibres only successive isoforms from the proposed pathway should coexist. However, jump fibres in which MyHC-1 is co-expressed with MyHC-2x/d have been describedrecently. The present study describes possible coexpression of MyHCI with MyHC-2b in transforming as well as in normal control mouse and rat musclefibres. The study is only descriptive and provides not sufficient proof to exclude the possible artefact resulting from unknown technical reasons.Source: Basic and applied myology. - ISSN 1120-9992 (Letn. 11, št. 3, 2001, str. 121-127)Type of material - article, component partPublish date - 2001Language - englishCOBISS.SI-ID - 15225305
Author
Eržen, Ida |
Sketelj, Janez |
Agbulut, Onnik |
Angaut-Petit, Denise |
Butler-Browne, Gillian S.
Topics
Muscle, skeletal |
Myosin heavy chains |
Analysis |
Classification |
Mice |
Rats, wistar |
Immunohistochemistry |
Electrophoresis |
Blotting, western |
Miozinske težke verige |
Mišica skeletna |
Blot, Western |
Elektroforeza |
Imunohistokemija |
Miši |
Podgane, Wistar
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Eržen, Ida | 05329 |
Sketelj, Janez | 04412 |
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