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  • Hi▫$s_6$▫-OPH enzyme-based bio-hybrid material for organophosphate detection [Elektronski vir]
    Frančič, Nina ...
    In this work, we report on the development of a bio-sensing film for the detection of organophosphorous compounds using sol-gel technology. A novel sol-gel immobilization method employing ... tetraethoxysilane/3- glycidoxypropyltrimethoxysilane/water hybrid material was developed and used to immobilize the hexahistidinetagged organophosphorous hydrolase enzyme (His6-OPH). Bio-sensing layers with encapsulated His6-OPH of various structures (water/silane, precursor ratios) have been prepared. The optimal (P=5:1, R=188) bio-sensing layers retained 90% of the initial enzyme activity. Furthermore, the bio-sensing layer prepared by this method was able to maintain its activity at or above 80% of its initial activity for 2 weeks. The bio-hybrid film also showed excellent reusability and improved activity atneutral pH in comparison to the same enzyme in solution.
    Source: Analytical and bioanalytical chemistry. - ISSN 1618-2642 (Vol. 401, no. 8, 2011, str. 2631-2638)
    Type of material - article, component part
    Publish date - 2011
    Language - english
    COBISS.SI-ID - 15269398
    DOI

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