Testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus, a filamentous fungus. The maximal androgen binding occurred by 120 min ...at 0°C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean ▫$K_d$▫ determined for testosterone binding protein (TBP) by Scatchard analysis was 4.5 nM and the maximum capacity ▫$B_{max}$▫ was 120 fmol/mg protein; for androstenedione binding protein (ABP) the ▫$K_d$▫ was 8 nM and ▫$B_{max}$▫ was 390 fmol/mg protein. Steroid binding specificity for TBP, in order of decreasing affinity, was the following: androstenedione, progesterone, dihydrotestosterone, 17 ▫$\beta$▫-estradiol and estrone. The same order was found for ABP.
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