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  • CRISPR/Cas-mediated plant genome editing : outstanding challenges a decade after implementation
    Cardi, Teodoro ...
    The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of ... specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants.
    Source: Trends in Plant Science. - ISSN 1360-1385 (Vol. 28, iss. 10, 2023, Str. 1144-1165)
    Type of material - article, component part
    Publish date - 2023
    Language - english
    COBISS.SI-ID - 156416259

    Link(s):

    https://www.cell.com/action/showPdf?pii=S1360-1385%2823%2900164-4
    Repository of the University of Ljubljana – RUL
    DOI

source: Trends in Plant Science. - ISSN 1360-1385 (Vol. 28, iss. 10, 2023, Str. 1144-1165)
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