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Vpliv steroidov na izražanje nekaterih genov glive = The effect of steroids on expression of some genes from Rhizopus nigricans : diplomska naloga
Fuekaš, Denis
Rhizopus nigricans je filamentozna gliva iz debla Zygomycota, ki se na prisotnost nekaterih steroidnih hormonov odzove z indukcijo steroidnega hidroksilaznega sistema, encima mutarotaze in indukcijo ...stresnih proteinov. Razen tega so steroidi za glivo tudi toksièni in zavirajo njeno rast.Pri nasemdelu smo se osredotoèili na preuèevanje izrazanje genov glive R. nigricansČ Rnhsp90-1, RnBiP-1 in RnRBP-1, ki so bili predhodno izolirani iz genomske knjiznice oz. iz knjiznice cDNA. Gena Rnhsp90-1 in RnBiP-1 vsebujeta zapisa za proteina, homologna stresnim proteinom, iz druzin Hsp90 oz. Hsp70, tako da sta lahko udelezena pri odzivu glive R. nigricans na steroidni stres. Gen RnRBP-1 pa vsebuje zapis za RNA vezavne proteine, ki uravnavajo izrazanje genov na posttranskripcijski ravni in bi lahko imel regulatorno vlogo pri odzivu glive na steroide.Tako smo analizirali ravni mRNA omenjenih genov v toku izpostavitve glive testosteronu, deoksikortikosteronu in progesteronu. Raven mRNA smo spremljali s hibridizacijo northern pri kateri smo celokupno RNA, vezano na najlonskih membranah, hibridizirali z radioaktivno oznaèenimi gensko specifiènimi sondami posameznih genov.
Type of material - undergraduate thesis
Publication and manufacture - Ljubljana : [Fuekaš, D.], 2004
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