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Melanin biosynthesis in the fungus Curvularia lunata (teleomorph: Cochlioboluslunatus)Lanišnik-Rižner, Tea ; Wheeler, Michael H.Curvularia lunata (teleomorph: Cochliobolus lunatus) is a known plant and human pathogen. Tricyclazole, a specific inhibitor of pentaketide melanin biosynthesis, blocked the biosynthesis of melanin ... in Curvularia lunata and caused the accumulation of the melanin metabolites flaviolin and 2-hydroxyjuglone. This showed that melanin in Curvularia lunata is produced bya pentaketide pathway from 1,8-dihydroxynaphthalene. The 1,3,8trihydroxynaphthalene reductase (3HNR) gene, associated with the melanin pathway of Curvularia lunata, was identified and characterized. An alignment of 3HNR sequences enabled the design of primers covering conserved regions. A PCR-amplified fragment of Curvularia lunata genomic DNA was used for screeningthe cDNA library. Three independent cDNA clones revealed an 801-bp open reading frame encoding a 267 amino acid protein. The protein was expressed in Escherichia coli and purified to homogeneity. The predicted aminoacid sequence of the 28.6-kDa protein demonstrated homology to other fungal 3HNR and other members of the short-chain dehydrogenase super family. Northern analyses revealed that 3HNR from Curvularia lunata is expressed synchronously with melanization after 3 days of Curvularia lunata growth in malt extract medium. No 3HNR reductase gene expression nor melanization was observed when Curvularia lunata was grown in yeast nitrogen base medium.Source: Canadian journal of microbiology = Journal canadien de microbiologie. - ISSN 0008-4166 (Letn. 49, 2003, str. 110-119)Type of material - article, component partPublish date - 2003Language - englishCOBISS.SI-ID - 16140761
Author
Lanišnik-Rižner, Tea |
Wheeler, Michael H.
Topics
Ascomycetes |
Metabolism |
Genetics |
Enzymology |
Melanins |
Biosynthesis |
Naphthols |
Oxidoreductases |
Dna, Fungal |
Polymerase Chain Reaction |
Gene Library |
Amino Acid Sequence |
Dna, Complementary |
Genome, Fungal |
Chromatography, Thin Layer |
Chromatography, High Pressure Liquid |
Blotting, Northern |
Spectrophotometry |
DNA glivična |
Melanini |
Naftoli |
Oksidoreduktaze |
Aminokislinsko zaporedje |
Blot, Northern |
DNA komplementarna |
Genom gliv |
Genska knjižnica |
Kromatografija tankoplastna |
Kromatografija visokotlačna, tekočinska |
Polimerazna, verižna reakcija |
Spektrofotometrija |
Curvularia Lunata |
Cochliobolus Lunatus
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Lanišnik-Rižner, Tea | 11699 |
Wheeler, Michael H. | ![]() |
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