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Candidate pathogenicity factor/effector proteins of ‘Candidatus Phytoplasma solani’ modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomes [Elektronski vir]Dermastia, Marina ...The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ... ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.Source: Frontiers in plant science [Elektronski vir]. - ISSN 1664-462X (Vol. 14, [art.] 1232367, 2023, str. 1-16)Type of material - e-articlePublish date - 2023Language - englishCOBISS.SI-ID - 161925891
Author
Dermastia, Marina |
Tomaž, Špela |
Strah, Rebeka |
Lukan, Tjaša |
Coll Rius, Anna |
Dušak, Barbara, biokemičarka |
Čepin, Timotej |
Kladnik, Aleš |
Zagorščak, Maja |
Gruden, Kristina |
Pompe Novak, Maruša
Topics
autophagosome |
effector |
glycolysis |
pathogenicity factor |
StAMP |
Candidatus Phytoplasma solani
Author | Dermastia, Marina ... |
Title | Candidate pathogenicity factor/effector proteins of ‘Candidatus Phytoplasma solani’ modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomes [Elektronski vir] |
Publication date | 2023-08-18 |
COBISS.SI-ID | 161925891 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Molekulske osnove interakcij med vinsko trto in fitoplazemskimi povzročiteljicami trsnih rumenic | J1-7151-2016 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Ciljana mutageneza s CRISPR/CAS9 za odpornost vinske trte in krompirja proti fitoplazmam | J4-2544-2020 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Molecular bases of interactions in grapevine yellow diseases | I 2763 |
Austrian Science Fund (FWF) |
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Resistance of grapevine and potato against phytoplasmas | I 5042 |
Austrian Science Fund (FWF) |
Files that belong to the publication
Link |
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https://doi.org/10.3389/fpls.2023.1232367 |
https://d.cobiss.net/repository/si/files/161925891/124066/fpls-14-1232367.pdf |
https://dirros.openscience.si/IzpisGradiva.php?id=16831 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Dermastia, Marina | 08280 |
Tomaž, Špela | 50566 |
Strah, Rebeka | 55750 |
Lukan, Tjaša | 36323 |
Coll Rius, Anna | 34130 |
Dušak, Barbara, biokemičarka | 51439 |
Čepin, Timotej | |
Kladnik, Aleš | 20070 |
Zagorščak, Maja | 39320 |
Gruden, Kristina | 12688 |
Pompe Novak, Maruša | 18467 |
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