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Hybrid capture II HPV test detects at least 15 human papillomavirus genotypes not included in its current high-risk probe cocktailPoljak, Mario, 1965- ...Background: Hybrid Capture II HPV Test (HCII) (Digene Corporation, Gaithersburg, MD) is a signal amplified hybridization microplate-based assay designated to detect 18 human papillomavirus (HPV) ... genotypes using two probe cocktails, for high-risk HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56,58, 59 and 68 and low-risk HPV genotypes 6, 11, 42, 43 and 44. At present, HCII is the only commercially available HPV assay with sufficient scientific data to support its performance in the clinical setting. Objectives: To determine the specificity and accuracy of HCII high-risk probe cocktail for detection of 13 HPV genotypes included in the high-risk probe cocktail. Study design: Cervical samples obtained from 325 women recognized as HPV positive using the HCII high-risk probe cocktail were included in the study. HPV genotypes were determined by restriction fragment analysis of PGMY09/PGMY11 polymerase chain reaction (PCR) products using seven restriction endonucleases. Results: A 450 bp fragment of HPV L1 gene was successfully amplified from 312 out of 325 samples. Of the 312 PCR-positive samples, 280 samples were associated with the expected high-risk HPV genotypes and 32 samples with the HPV genotypes not included in the HCII high-risk probe cocktail. Thus, HPV53 was detected in 8 samples, HPV66 in 4 samples, HPV54 in 3 samples, HPV6, HPV26, HPV70 each in 2 samples, and HPV11, HPV40, HPV42, HPV61, HPV73, HPV81, MM4, IS39, CP6108 each in 1 sample. In 2 samples, we werenot able to determine the HPV genotype. Conclusions: Our study shows that HCII high-risk probe cocktail detects at least 15 HPV genotypes not included in the current HCII high-risk probe cocktail. The potential impact of HCII high-risk probe cocktail cross-reactivity with phylogenetically related and unrelated HPV genotypes, including genotypes currently considered to be low-risk HPVs, remains to be determined.Source: Journal of clinical virology. - ISSN 1386-6532 (Letn. 25, suppl. 3, 2002, str. S89-97)Type of material - article, component partPublish date - 2002Language - englishCOBISS.SI-ID - 16392409
Author
Poljak, Mario, 1965- |
Marin, Irena J. |
Seme, Katja, 1966- |
Vince, Adriana
Topics
Tumor Virus Infections |
Virology |
Papillomavirus, Human |
Isolation And Purification |
Genetics |
Classification |
Papovaviridae Infections |
Virology |
Reagent Kits, Diagnostic |
Cross Reactions |
Dna Probes, Hpv |
Dna, Viral |
Genetics |
Analysis |
Molecular Probe Techniques |
Genotype |
Risk Factors |
Sensitivity And Specificity |
Papilomavirus človeški |
Papovaviridne infekcije |
Reagentni kompleti diagnostični |
Tumorski virusi, infekcije |
DNA sonde, HPV |
DNA virusna |
Dejavniki tveganja |
Genotip |
Molekularna sonda, tehnike |
Navzkrižne reakcije |
Občutljivost in specifičnost
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Database name | Field | Year |
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Poljak, Mario, 1965- | 11622 |
Marin, Irena J. | 19131 |
Seme, Katja, 1966- | 14504 |
Vince, Adriana | ![]() |
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