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The evaluation of laccase-catalysed oxidation of lignin isolated from lignocellulosic fibres [Elektronski vir]
Krajnc, Helena ...
In these study lignins from different lignocellulosic fibres (jute, sisal and coconut) were used. Different preparation (wax, ethanol/water solubles and pectic substances removal) procedures were ...used to achieve the best conditions for lignin analysis. Lignin was isolated using modified soda pulping treatment followed by enzymatic hydrolysis to achieve the highest purity and structure integrity as possible. Enzymatic modification of isolated lignins was further performed using two different types of laccases, i.e. bacterial and fungal origin. Differences in lignin structure before and after the enzymatic treatment were evaluated with spectroscopic methods as ATR-FTIR, UV-VIS and XPS spectroscopy. Additionally, average particle size and surface charge of lignins were evaluated.
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