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  • Patient-derived organoids mimic glioblastoma resistance to therapy and increase DNA Damage Response [Elektronski vir]
    Majc, Bernarda ...
    Glioblastoma (GB) is the most common primary malignant brain tumour characterised by resistance to therapy. Despite aggressive treatments, GB remains an incurable disease. Invasiveness and ... heterogeneity are key GB features that lack to be retained in preclinical in vitro models. In this study, we investigated the effect of standard therapy by using patient-derived GB organoids (GBOs). GBOs reflect the complexity and heterogeneity of the original tumour tissue. No significant effect on GBO viability or invasion was observed. The E3 ubiquitin-protein ligase (MDM2), cyclin-dependent kinase inhibitor 1A (CDKN1A), and serine/threonine kinases ATM and ATR were upregulated at the gene and protein levels after treatment. Our results show that the p53 pathway and DNA damage response mechanisms were triggered, suggesting that GBOs recapitulate GB therapy resistance. Patient-derived GBOs thus provide a highly efficient platform to evaluate the specific responses of GB patients to therapy and to further explore therapy resistance.
    Source: iScience [Elektronski vir]. - ISSN 2589-0042 (Vol. , no. , 2023, str. [1-10])
    Type of material - e-article ; adult, serious
    Publish date - 2023
    Language - english
    COBISS.SI-ID - 167651587
    DOI

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