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Enumration, isolation, and identification of bifidobacteria from dairy products = Štetje, izolacija in identifikacija bifidobakterij v mlečnih izdelkih
Vlková, Eva ; Rada, Vojtěch ; Trojanová, Iva
Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l-1). Isolates were identified to the genus level by the ...detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers.
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