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Improvements in protoplast isolation protocol and regeneration of different cabbage (Brassica oleracea var. capitata L.) cultivars [Elektronski vir]Stajič, EsterProtoplasts are a versatile tool in plant biotechnology since they can be used for basic biological studies as well as for breeding strategies based on genome editing. An efficient protoplast ... isolation protocol is essential for conducting protoplast-based studies. To optimize the protoplast isolation protocol in cabbage (Brassica oleracea var. capitata L.), different enzyme solutions were tested for the isolation of leaf mesophyll protoplasts. In our experiments, the combination of 0.5% Cellulase Onozuka RS and 0.1% Macerozyme R-10 showed the best result. The optimized protocol proved suitable for the isolation of protoplasts from five different cabbage cultivars with yields ranging from 2.38 to 4.63 × 106 protoplasts/g fresh weight (fw) and a viability of 93% or more. After three weeks in culture, protoplasts from all of the tested cultivars formed micro-calli, but further callus growth and shoot regeneration depended strongly on the genotype and regeneration protocol used. For shoot formation, 1 mg/L BAP in combination with auxin 0.2 mg/L NAA showed the best results with a regeneration of 23.5%. The results obtained will contribute to the development of different applications of cabbage protoplasts and facilitate the breeding process of this important horticultural crop.Source: Plants [Elektronski vir]. - ISSN 2223-7747 (Vol. 12, iss. 17, art. 3074, 2023, 12 str.)Type of material - e-article ; adult, seriousPublish date - 2023Language - englishCOBISS.SI-ID - 168164355
Author
Stajič, Ester
Topics
Brassica oleracea |
protoplasts |
isolation |
regeneration |
protocol |
cabbage |
cultivars |
zelje |
izolacija protoplastov |
protokol |
regeneracija |
izolacija
Author | Stajič, Ester |
Title | Improvements in protoplast isolation protocol and regeneration of different cabbage (Brassica oleracea var. capitata L.) cultivars [Elektronski vir] |
Publication date | 2023-08-27 |
COBISS.SI-ID | 168164355 |
Publication version in repository | Publisher's version |
Publication licence | Creative Commons Attribution 4.0 International |
Embargo | Immediate publication for public |
Project(s) from which the publication was funded
Title | Acronym | Project ID | Funder |
---|---|---|---|
Optimizacija protokola za CRISPR/Cas9 in tarčno preurejanje nukleotidov pri zelju | Z4-3215-2021 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Kmetijske rastline - genetika in sodobne tehnologije | P4-0077-2019 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Files that belong to the publication
Link |
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https://www.mdpi.com/2223-7747/12/17/3074 |
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=151618 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Stajič, Ester | 38120 |
Source: Personal bibliographies
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